Trifluorothymidine
Thymidylate synthase inhibitor; Anti-cancer agent / An analog of thymidine which inhibits thymidylate synthase possesses antiviral and anticancer activity.1,2 After phosphorylation by thymidine kinase, it is incorporated into DNA where it induces DNA-damage and interferes with repair enzymes.3 Enhances frame shift insertion and deletion in CRISPR genome editing in pluripotent stem cells.4
Biochemicals & reagents
70-00-8
α,α,α-Trifluorothymidine; Trifluorothymine deoxyriboside; Trifluridine; TFT
1) Bijnsdorp et al. (2010), Differential activation of cell death and autophagy results in an increaded cytotoxic potential for trifluorothymidine compared to 5-fluorouracil in colon cancer cells; Int.J. Cancer, 126 2457 2) Temmink et al. (2010), Trifluorothymidine resistance is associated with decreased thymidine kinase and equilibrative nucleoside transporter expression or increased secretory phospholipase A2; Mol. Cancer Ther., 9 1047 3) Suzuki et al. (2011), Mode of action of trifluorothymidine (TFT) against DNA replication and repair enzymes; Int. J. Oncol., 39 263 4) Yu et al. (2015), Small molecules enhance CRISPR genome editing in pluripotent stem cells; Cell Stem Cell, 16 142
-20°C
TARGET: DNA -- PATHWAY: DNA damage -- RESEARCH AREA: Cancer Stem Cells; CRISPR -- DISEASE AREA: Cancer; Infectious disease