Hypoxanthine
Nutrient additive for cell culture / Hypoxanthine is a natural purine analog and a breakdown product of adenosine.1 Together with aminopterin and thymidine, it is a component of HAT medium2, which is used to select clones during recombinant protein and antibody production, particularly important in bioprocessing. It is a biomarker of ischemia2,3, and it is used in malaria research as an essential nutrient for P. Falciparum4. It also can enable stem cell expansion.5
Biochemicals & reagents
68-94-0
1 Chen and Sorgensen (1956), Formation of hypoxanthine from adenosine triphosphate in shed human blood; Acta Pharmacol. Toxicol. (Copenh), 12 369 2 Migeon and Miller (1968), Human-mouse somatic cell hybrids with single human chromosome (group E): link with thymidine kinase activity; Science 162 1005 3 Von Holst and Sollevi (1985), Increased concentration of hypoxanthine in human central cerebrospinal fluid after subarachnoid haemorrhage; Acta Neurochir. (Wien) 77 52 4 Tewari et al. (2019), Short-term metabolic adjustments in Plasmodium falciparum counter hypoxanthine deprivation at the expense of long-term viability; Malar. J. 18 Article number 86 5 Huhi et al. (2011), SACK-expanded hair follicle stem cells display asymmetric nuclear Lgr5 expression with non-random sister chromatid segregation; Sci. Rep. 1 176
-20°C
TARGET: DNA -- PATHWAY: DNA damage; Nucleoside/tide biosynthesis -- RESEARCH AREA: Neuroscience; Stem cells -- DISEASE AREA: Ischemia; Infectious disease