PODS™ Human FGF-7 crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically, using 50 million PODS™ crystals equivalence to 3.3 µg of standard growth factor is a good starting point. To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.
- Applications:cell culture growth factors
- Applications:PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting. PODS™ protein crystals are highly stable when stored in aqueous solution (pH
Peptides & proteins
Fibroblast Growth Factor 7, FGF7, FGF 7, KGF, Keratinocyte Growth Factor
1) Ijiri, H. et al. Structure-based targeting of bioactive proteins into cypovirus polyhedra and application to immobilized cytokines for mammalian cell culture. (2009) Biomaterials. 30(26): 4297-4308.; 2) Shimabukuro, J. et al. 3D co-cultures of keratinocytes and melanocytes and cytoprotective effects on keratinocytes against reactive oxygen species by insect virus-derived protein microcrystals. (2014) Materials and Science Engineering. 42: 64-69.; 3) Coulibaly, F. et al. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.; 4) Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.; 5) Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.; 6) Abe, S. et al. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.
Upon receipt, store at 4°C. PO
SOURCE: Spodoptera frugiperda (Sf9) cell culture; LENGTH: 208 aa; STRUCTURE: Monomer; FORMAT: lyophilized; ENDOTOXIN LEVEL: <0.06 EU/ml as measured by gel clot LAL assay; BACKGROUND: Fibroblast Growth Factor 7 (FGF-7), also known as Keratinocyte Growth Factor (KGF), is a potent mitogen that regulates epithelial cell migration and differentiation. FGF-7 is produced by mesenchymal cells and binds in high affinity to a splice variant of FGF receptor 2 (FGFR2-IIIb). The mitogenic activity of FGF-7 acts predominantly on keratinocytes, but not on fibroblast or endothelial cells. FGF-7 expression is upregulated after acute and chronic injury, suggesting that FGF-7 functions during the healing of injured epithelial cells. FGF-7 also induces the formation of the apical ectoderm ridge during limb development.