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PODS Growth Factors
iclines

PODS™ Human RANK Ligand

PODS™ Human RANK Ligand
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Size: 1 billion
$1600.00
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Catalogue number:
PPH19-1000
Name:
PODS™ Human RANK Ligand
Supplier:
Availability:

PODS™ Human RANK Ligand crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically, using 50 million PODS™ crystals equivalence to 3.3 µg of standard growth factor is a good starting point. To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.

Applications:

PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting. PODS™ protein crystals are highly stable when stored in aqueous solution (pH

Product Type:
Peptides & proteins
Alternative Names:
Receptor activator of nuclear factor kappa-B Ligand, RANKL, RANK-L, RANK-ligand, TNFSF11, TRANCE, OPGL, ODF
Storage Temp:
4°C
Antibody Type:
cell culture growth factors
Limit of Detection:
25 kDa
Range:
1) Coulibaly, F. et al. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.; 2) Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.; 3) Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.; 4) Abe, S. et al. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.
Shelf Life:
Upon receipt, store at 4°C. PO
Additional Information:
SOURCE: Spodoptera frugiperda (Sf9) cell culture; LENGTH: 221 aa; STRUCTURE: Monomer; FORMAT: lyophilized; ENDOTOXIN LEVEL: <0.06 EU/ml as measured by gel clot LAL assay; BACKGROUND: Receptor activator of nuclear factor kappa-B Ligand (RANK Ligand) is a cell-bound marker related to the Tumor Necrosis Factor (TNF) family of proteins. RANK Ligand plays a critical role in bone metabolism and osteoclast differentiation. T cell expression of RANK Ligand promotes dendritic cell maturation.