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Pluripro
PODS Growth Factors
iclines

PODS™ Human SHH

PODS™ Human SHH
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Size: 250 million
$480.00
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Catalogue number:
PPH168-250
Name:
PODS™ Human SHH
Supplier:
Availability:

PODS™ Human SHH crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically, using 50 million PODS™ crystals equivalence to 3.3 µg of standard growth factor is a good starting point. To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.

Applications:

PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting. PODS™ protein crystals are highly stable when stored in aqueous solution (pH

Product Type:
Peptides & proteins
Alternative Names:
Sonic hedgehog, HHG-1, HHG1, HLP3, HPE3
Storage Temp:
4°C
Antibody Type:
cell culture growth factors
Limit of Detection:
25 kDa
Range:
1) Coulibaly, F. et al. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.; 2) Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.; 3) Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.; 4) Abe, S. et al. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.
Shelf Life:
Upon receipt, store at 4°C. PO
Additional Information:
SOURCE: Spodoptera frugiperda (Sf9) cell culture; LENGTH: 220 aa; STRUCTURE: Monomer; FORMAT: lyophilized; ENDOTOXIN LEVEL: <0.06 EU/ml as measured by gel clot LAL assay; BACKGROUND: Sonic hedgehog (SHH) is a member of a small group of hedgehog secreted proteins that are essential for development in both vertebrates and invertebrates. There are three mammalian hedgehog homologues, sonic, desert, and indian, that signal via the Patched-1 and Patched-2 receptors. SHH is a morphogen that is essential during vertebrate organogenesis and adult stem cell division.