Recombinant Human MIF (migration inhibitory factor) acts as an inflammatory mediator consisting of two alpha-helices and six beta-strands, four of which form a beta-sheet structure. The remaining two beta-strands interact with other MIF molecules forming a trimer in solution. Human MIF acts on fibroblasts by inducing IL-1, IL-8 and MMP expression. MIF stimulates NO production and TNF-alpha release following IFN-gamma activation on macrophages. Human MIF is a non-glycosylated polypeptide.