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LIF

LIF
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Size: 100 μg
$300.00
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Catalogue number:
GFM200-100
Name:
LIF
Supplier:
Cell Guidance Systems
Availability:

Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of pluripotent embryonic stem cells by suppressing spontaneous differentiation. Recombinant Mouse Leukemia inhibitory factor (mLIF) is produced in E. coli. It contains a single non-glycosylated polypeptide chain 181 amino acids and has a molecular mass of 20kDa. mLIF is purified by proprietary techniques using HPLC and FPLC chromatography. The sample purity is better than 99% on SDS page. Its biological activity has been determined.

Data sheet:

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Product Type:
Peptides & proteins
Alternative Names:
Leukocyte Inhibitory Factor
Storage Temp:
-20°C
Molecular Weight:
20 kDa
Specifications:
PURITY (MIN.): ≥95
UNSPSC Code:
12352202
Additional Information:
NAME: Leukemia Inhibitory Factor; ACCESSION/UNIPROT#: P09056; EXPRESSION SYSTEM: E.coli; FORMAT: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA). Reconstitute in sterile 10 mM acetic acid at 0.1 mg/mL.; TESTING: Induce IL-6 production from M1 cells with an acceptance criteria of 1 ng/mL (ED50), 1 x 10^6 units/mg.; #AA: 181; SEQUENCE: MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F