TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF. Recombinant Human Tumor Necrosis Factor-alpha is a single, non-glycosylated polypeptide.
Peptides & proteins
TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF
NAME: Tumor Necrosis Factor alpha; ACCESSION/UNIPROT#: P01375; EXPRESSION SYSTEM: E.coli; FORMAT: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5. Reconstitute in sterile water at 0.1 mg/mL.; TESTING: Cytolysis of mouse L929 cells in the presence of Actinomycin D with an acceptance criteria of 2 ng/mL (ED50), 5 x 10^5 units/mg.; #AA: 158; SEQUENCE: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL