- In this section:
- ChIP Positive and Negative Primer Sets
The use of high quality and specific ChIP validated antibodies is essential for the success of a ChIP assay. The antibody must recognise and bind to native protein that is bound to DNA. Antibodies validated in other applications do not always work well in ChIP.
Chromatrap® ChIP-validated Antibodies can now be supplied with matching primer sets to cover all aspects of ChIP validation.
- Reduce time spent searching databases and designing primers
- Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
- Have confidence that the primers are tested with the exact antibody and gene target
Many common epigenetic targets of interest are now available as combined antibody & primer sets. Forward and reverse primers are also available separately for common targets.
Ordering
Target | Antibody | w/ Positive Primers | w/ Positive & Negative Primers |
---|---|---|---|
5mC | 700005 | 900011 | 900000 |
AcH3 | 700006 | 900013 | -- |
AcH4 | 700007 | 900018 | -- |
AR | 700008 | 900019 | 900006 |
H2Az | 700019 | 900034 | -- |
H3 (Ms host) | 700000 | 900010 | -- |
H3 (Rb host) | 700020 | 900033 | -- |
H3K14ac | 700012 | 900022 | 900008 |
H3K27ac | 700011 | 900021 | 900009 |
H3K4me1 | 700016 | 900028 | 900023 |
H3K4me3 | 700010 | 900014 | 900002 |
H3K9me1 | 700022 | 900031 | 900026 |
H3K9me2 | 700023 | 900032 | 900027 |
H3K9me3 | 700003 | 900017 | 900005 |
H3K27me3 | 700013 | 900012 | 900001 |
H4 | 700021 | 900035 | -- |
H4K5ac | 700017 | 900029 | 900024 |
H4K12ac | 700018 | 900030 | 900025 |
RNA pol II (S) | 700001 | 900015 | 900003 |
RNA pol II (S5) | 700002 | 900016 | 900004 |
Sin3a | 700009 | 900020 | 900007 |
Rabbit IgG (Control) | 700014 | -- | -- |
Sheep IgG (Control) | 700015 | -- | -- |