Human Control qPCR Primer Sets are designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with human samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR of ChIP samples from multiple human cell lines.
Positive Primer Sets
| Cat# | Set | Gene | Targeting |
|---|---|---|---|
| 800000 | Set 1 | GAPDH | H3K4me1, H3K4me3, H3K27ac, RNA Pol II, etc. |
| 800001 | Set 2 | H19 | 5mC |
| 800002 | Set 3 | Myt1 | H3K27me3, EZH2 |
| 800003 | Set 4 | SAT2 | H3K9me3 |
| 800004 | Set 5 | FkBP5 | AR |
| 800005 | Set 6 | MLH1 | Sin3a |
| 800006 | Set 7 | B-Globin | H3K14ac, RNA Pol II |
| 800007 | Set 8 | IL-8 | NF-kB |
| 800013 | Set 9 | GREB1 | H4K12ac |
Negative Primer Sets
| Cat# | Set | Gene | Targeting |
|---|---|---|---|
| 800008 | Set 1 | UBE2B | 5mC |
| 800009 | Set 2 | GAPDH | Sin3a H3K4me1, H3K4me3, H3K27ac, RNA Pol I |
| 800010 | Set 3 | B-Globin | AR |
| 800011 | Set 4 | SAT2 | H3K14ac |
| 800012 | Set 5 | Myt1 | H3K27ac |
| 800014 | Set 6 | GAPDH | H3K4me1 |
| 800015 | Set 7 | c-fos | H3K9me1 |
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