PODS™ Empty crystals display the same physical properties as other PODS™ growth factor products. While PODS™ Empty behave in the same way as other PODS crystals, they differ in that they do not contain or release cargo protein and serve as a negative control for PODS validation experiments. They can be used analogous to other PODS growth factor products.
- Applications:cell culture growth factors
- Applications:PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting. PODS™ protein crystals are highly stable when stored in aqueous solution (pH
Peptides & proteins
Bombyx mori cypovirus polyhedrin protein
1) Coulibaly, F. et al. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.; 2) Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.; 3) Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.; 4) Abe, S. et al. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.
Upon receipt, store at 4°C. PO
SOURCE: Spodoptera frugiperda (Sf9) cell culture; LENGTH: 252 aa; FORMAT: lyophilized; ENDOTOXIN LEVEL: <0.06 EU/ml as measured by gel clot LAL assay; BACKGROUND: PODS™ Empty crystals are composed solely of polyhedrin protein which self-assembles into regular, cubic crystals. Thus, these PODS™ crystals do not contain any cargo protein, in contrast to other PODS™ growth factor products, and are intended to be used as negative controls alongside cargo-containing PODS™ crystals. The cross-reactivity of PODS™ Empty crystals has been tested on a variety of cells including mouse ES cells, PC12, fibroblasts, and ETS embryos, and there was no observable interference with the tested cells. Additionally, in-vivo data indicate no inflammatory response to PODS™ Empty crystals in animals.