Blebbistatin
Non-muscle myosin II inhibitor / Blebbistatin is a potent, selective, reversible, cell-permeable inhibitor of non-muscle myosin II (IC50 ~4 mM).1,2 During cell division it inhibits contraction of the cleavage furrow without disrupting mitosis.1 Blocks cell blebbing (hence its name) and rapidly disrupts directed cell migration and cytokinesis in many cell types.1 It promotes neurite outgrowth3 and is neuroprotective against vincristine-induced neurotoxicity in iPSC-derived neurons4.
Biochemicals & reagents
674289-55-5
1 Straight et al. (2003), Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor; Science 299 1743 2 Kovacs et al. (2004), Mechanism of blebbistatin inhibition of myosin II; J. Biol. Chem. 279 35557 3 Yu et al. (2012), Myosin II activity regulates neurite outgrowth and guidance in response to chondroitin sulfate proteoglycans; J. Neurochem. 120 1117 4 Petrova et al. (2024), Identification of novel neuroprotectants against vincristine-induced neurotoxicity in iPSC-derived neurons; Cell Mol. Life Sci. 81 315
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TARGET: Cytoskeleton -- PATHWAY: Cell cycle; Cell migration; Cell adhesion -- RESEARCH AREA: Stem cells; Neuroscience -- DISEASE AREA: Neurodegeneration; Cancer