gamma-L-Glutamyl-p-nitroanilide HCl
L-glutamine uptake inhibitor and γ-glutamyl transpeptidase substrate / L-γ-Glutamyl-p-nitroanilide (GPNA) is a substrate for γ-glutamyl transpeptidase. GPNA is used for the determination of γ-glutamyl transpeptidase hydrolytic activity via spectrophotometric monitoring of p-nitroaniline released by the action of the enzyme on GPNA.1 GPNA has also been shown to be an inhibitor of glutamine uptake – solute-linked carrier family A1 member 5 (SLC1A5)2 Cellular uptake of L-glutamine (regulated by SLC1A5) is a rate-limiting step that activates mTOR and is, thus, an interesting cancer chemotherapy target.3
Biochemicals & reagents
67953-08-6
GPNA
1) Meister et al. (1981), γ-Glutamyl transpeptidase; Methods Enzmol. 77 237 2) Esslinger et al. (2005), N-gamma-aryl glutamine analogues as probes of the ASCT2 neutral amino acid transporter binding site; Bioorg.Med.Chem. 13 1111 3) Nicklin et al. (2012), Bidirectional Transport of Amino Acid Regulates mTOR and Autophagy; Cell 136 521
-20°C
TARGET: Glutathione; Substrate / Probe -- PATHWAY: mTOR; Amino acid metabolism -- RESEARCH AREA: Neuroscience -- DISEASE AREA: Cancer