4-MUG
β-Galactosidase fluorogenic substrate / Versatile fluorogenic substrate for ß-galactosidase1 for use in cell extracts or purified enzyme preparations.1-3 May be used as a fluorescent probe for identification of senescent cells based on lysosomal ß-galactosidase activity.4,5 This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm
Biochemicals & reagents
6160-78-7
4-MU-b-D-Gal; MUgal; MUG; MUGA
1 Chiu et al. (2017), Measuring ß-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter; Curr. Protoc. Toxicol., Supp 74 4.44.1 2 Kytidou et al. (2018), Nicotiana benthamiana α-galactosidase A1,1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease; J. Biol. Chem., 293 10042 3 Hernandez-Guzman et al. (2016), Purification and characterization of an extracellular ß-glucosidase from Sporothrix schenckii; FEBS Open Bio., 6 1067 4 Lee et al. (2006), Senescence-associated beta-galactosidase is lysosomal beta-galactosidase; Aging Cell, 5 187 5 Sosinska et al. (2014), Specificity of cytochemical and fluorescence methods of senescence-associated ß-galactosidase detection for ageing driven by replication and time; Biogerontology, 15 407
-20°C / Protect from light
TARGET: Galactosidase; Substrate / Probe -- PATHWAY: Senescence