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ChIP Assay Reagents and Buffers

The success of a ChIP assay is highly dependent on the quality of chromatin prepared. Chromatrap® reagents are optimized for the isolation of quality chromatin from your sample. Product categories:

  • Lysis and Hypotonic Buffer for Sonication
  • Lysis Buffer for Enzymatic Shearing
  • Lysis Buffer for Sonication
  • Hypotonic Buffer

buffers

Lysis and Hypotonic Buffer for Sonication

For complete disruption of cytoplasmic and nuclear membranes, Chromatrap’s unique optimized buffer chemistry provides the solution. The detergent-based buffers are ideal for effective fragmentation of nuclear material by sonication for a range of different species and difficult to lyse cell types and tissues. Correct shearing is critical to the success of a ChIP assay. These lysis and hypotonic buffers for sonication are compatible with all of Chromatrap ChIP kits for sonication from fixed cells or tissue.

Product

Lysis and Hypotonic Buffer for Sonication

10 ml

100004

50 ml

100008

Lysis Buffer for Enzymatic Shearing

Chromatrap lysis buffer for enzymatic shearing provides users with a complete lysis buffer for the disruption of nuclear cell membranes with a milder detergent than that required for mechanical fragmentation. Correct shearing is critical to the success of a ChIP assay. The buffer is compatible with all Chromatrap ChIP kits for enzymatic shearing from fixed cells or tissue.

Product

Lysis Buffer for Enzymatic Shearing

10 ml

100002

50 ml

100006

Lysis Buffer for Sonication

Chromatrap Lysis buffer for sonication provides users with a detergent based buffer ideal for the fragmentation of samples by sonication. The optimized buffer composition ensures effective nuclear lysis for a range of different species and difficult to lyse cell types and tissues. Correct shearing is critical to the success of a ChIP assay. The buffer is compatible with all Chromatrap ChIP kits for sonication from fixed cells or tissue.

Product

Lysis Buffer for Sonication

10 ml

100001

50 ml

100005

Hypotonic Buffer

For the disruption of cell membranes, the optimized buffer composition ensures effective lysis of cell membranes to aid in the release of nuclei material for a range of different species.

Product

Hypotonic Buffer

10 ml

100003

50 ml

100007