ChIP Assay Reagents and Buffers

Lysis and Hypotonic Buffer for Sonication

For complete disruption of cytoplasmic and nuclear membranes, Chromatrap’s unique optimized buffer chemistry provides the solution. The detergent-based buffers are ideal for effective fragmentation of nuclear material by sonication for a range of different species and difficult to lyse cell types and tissues. Correct shearing is critical to the success of a ChIP assay. These lysis and hypotonic buffers for sonication are compatible with all of Chromatrap ChIP kits for sonication from fixed cells or tissue.

Lysis Buffer for Enzymatic Shearing

Chromatrap lysis buffer for enzymatic shearing provides users with a complete lysis buffer for the disruption of nuclear cell membranes with a milder detergent than that required for mechanical fragmentation. Correct shearing is critical to the success of a ChIP assay. The buffer is compatible with all Chromatrap ChIP kits for enzymatic shearing from fixed cells or tissue.

Lysis Buffer for Sonication

Chromatrap Lysis buffer for sonication provides users with a detergent based buffer ideal for the fragmentation of samples by sonication. The optimized buffer composition ensures effective nuclear lysis for a range of different species and difficult to lyse cell types and tissues. Correct shearing is critical to the success of a ChIP assay. The buffer is compatible with all Chromatrap ChIP kits for sonication from fixed cells or tissue.

Hypotonic Buffer

For the disruption of cell membranes, the optimized buffer composition ensures effective lysis of cell membranes to aid in the release of nuclei material for a range of different species.