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Catalogue number:
5 mg
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Protease inhibitor: Cysteine proteases; Autophagy modulator. Blocks entry of SARS-CoV-2 into cells that cannot express TMPRSS2. Hoffmann et al. bioRxiv, Jan 31 2020, https://doi.org/10.1101/2020.01.31.929042.

Product Type:

Biochemicals & reagents

CAS Number:



1) McGowan et al. (1989), Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d; Biochem. Biophys. Res. Commun., 158 432 2) Wilcox and Mason (1992), Inhibition of cysteine proteinases in lysosomes and whole cells; Biochem. J., 285 495 3) Mizushima et al. (2010), Methods in mammalian autophagy research; Cell, 140 313

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E-64d is a cell-permeable derivative of E-64c, an inhibitor of calpain and other cysteine proteases, such as papain, cathepsin B and cathepsin L (1,2). Addition of this compound to cell cultures should be done in serum-free media as esterases in serum will cleave the ethyl ester and reduce cell permeability using a typical working concentration is 0.5-10 µg/ml. It also inhibits degradation of autophagic cargo inside autolysosomes (3).