PODS™ Mouse VEGF-165 crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically, using 50 million PODS™ crystals equivalence to 3.3 µg of standard growth factor is a good starting point. To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.
- Applications:cell culture growth factors
- Applications:PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting. PODS™ protein crystals are highly stable when stored in aqueous solution (pH
Peptides & proteins
Vascular Endothelial Growth Factor, VEGF164, VEGF-A, VPF, glioma-derived endothelial cell mitogen
1) Matsumoto, G. et al. Control of angiogenesis by VEGF and endostatin-encapsulated protein microcrystals and inhibition of tumor angiogenesis. (2014) Biomaterials. 35(4): 1326-1333.; 2) Coulibaly, F. et al. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.; 3) Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.; 4) Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.; 5) Abe, S. et al. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.
Upon receipt, store at 4°C. PO
SOURCE: Spodoptera frugiperda (Sf9) cell culture; LENGTH: 210 aa; STRUCTURE: Dimer; FORMAT: lyophilized; ENDOTOXIN LEVEL: <0.06 EU/ml as measured by gel clot LAL assay; BACKGROUND: Vascular Endothelial Growth Factor A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-164 being the most abundant. The VEGF-164 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.