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PODS Growth Factors
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PODS™ Human BMP-4

PODS™ Human BMP-4
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Size: 1 billion
$1600.00
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Catalogue number:
PPH56-1000
Name:
PODS™ Human BMP-4
Supplier:
Availability:

PODS™ Human BMP-4 crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS™ crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically, using 50 million PODS™ crystals equivalence to 3.3 µg of standard growth factor is a good starting point. To control for cross-reactivity with cells or as a negative control, we recommend using PODS™ growth factors alongside PODS™ Empty crystals, as the latter do not contain or release cargo protein.

Applications:

PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting. PODS™ protein crystals are highly stable when stored in aqueous solution (pH

Product Type:
Peptides & proteins
Alternative Names:
Bone morphogenetic protein 4, bone morphogenetic protein 4A, BMP-4A, BMP4, BMP4A
Storage Temp:
4°C
Antibody Type:
cell culture growth factors
Limit of Detection:
103 kDa
Range:
1) Coulibaly, F. et al. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.; 2) Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.; 3) Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.; 4) Abe, S. et al. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.
Shelf Life:
Upon receipt, store at 4°C. PO
Additional Information:
SOURCE: Spodoptera frugiperda (Sf9) cell culture; LENGTH: 452 aa; STRUCTURE: Dimer; FORMAT: lyophilized; ENDOTOXIN LEVEL: <0.06 EU/ml as measured by gel clot LAL assay; BACKGROUND: Bone Morphogenic Protein 4 (BMP-4) is a member of the Bone Morphogenetic Protein (BMP) family. These proteins are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. BMP proteinsstimulate the production of bone matrix proteins and osteoclasts proliferation, and can be found on tissues related with bone or cartilage growth.