Zebrafish IL-10 Do-It-Yourself ELISA. Do-It-Yourself ELISA Kits include target capture antibody, protein standard, and detection antibody for development of a target-specific ELISA. Antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined so a working knowledge of ELISA is recommended. Compontent quantities are not matched but should allow for approximately ten 96 well plate assays. Additional components may also be purchased separately. Accessory reagents are also available for DIY ELISA Kits. These include TMB Substrate and Stop Solution (Cat# AR0133-002), Streptavidin-HRP (Cat# AR0068-001) and ELISA Plates (Cat# AR0658-005).
Assays & kits