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Green PIP2 Sensor BacMam Kit (CMV promoter)

Green PIP2 Sensor BacMam Kit (CMV promoter)
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Size: 10 ml kit
$595.00
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Catalogue number:
D0400G
Name:
Green PIP2 Sensor BacMam Kit (CMV promoter)
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Live cell assay. The green PIP2 sensor is based on a dimerization-dependent fluorescent protein and optimized for imaging rapidly dividing, immortalized cell lines. Green fluorescence decrease in intensity when PIP2 levels decrease. Packaged in BacMam, a BSL-1 insect virus engineered with a mammalian promoter, for safe handling and expression in a wide range of cell types. The fluorescent sensor used in the assay can be combined with different colored sensors, such as the red GECO calcium sensor or a red DAG sensor, to measure multiple signals simultaneously.

Applications:

live cell assay, PIP2 hydrolysis measurements
For ~400 dp (96 well), 1350+ dp (384 well).

Product Type:
Assays & kits
Storage Temp:
4° C
Reference:
1) Alford, S.C., Abdelfattah, A.S., Ding, Y., and Campbell, R.E. (2012). A fluorogenic red fluorescent protein heterodimer. Chem. Biol. 19, 353–360.; 2) Tewson et al. (2013). A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells. Journal of Biomolecular Screening 18, 797–806.; 3) Ding et al. (2015) Ratiometric biosensors based on dimerization dependent fluorescent protein exchange. Nature Methods, doi:10.1038/nmeth.3261
Additional Information:
VECTOR: BacMam; PROMOTER: CMV