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Red PIP2 Sensor BacMam Kit (CMV promoter)

Red PIP2 Sensor BacMam Kit (CMV promoter)
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Size: 10 ml kit
$595.00
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Catalogue number:
D0400R
Name:
Red PIP2 Sensor BacMam Kit (CMV promoter)
Supplier:
Availability:

Live cell assay. PIP2 is a red, dimerization-dependent fluorescent protein sensor under the control of a CMV promoter. When PIP2 is hydrolyzed, red fluorescence decreases. Packaged in BacMam, a BSL-1 insect virus engineered with a mammalian promoter, for safe handling and expression in a wide range of cell types. This sensor can be multiplexed with our green sensors for cAMP or DAG. When co-expressed with a green dimerization dependent monomer, GA, this sensor becomes a fluorescent protein exchange (FPX) sensor that increases in green fluorescence as the red fluorescence decreases.

Applications:

live cell assay, PIP2 hydrolysis measurements
For ~400 dp (96 well), 1350+ dp (384 well).

Product Type:
Assays & kits
Storage Temp:
4° C
Reference:
1) Alford, S.C., Abdelfattah, A.S., Ding, Y., and Campbell, R.E. (2012). A fluorogenic red fluorescent protein heterodimer. Chem. Biol. 19, 353–360.; 2) Tewson et al. (2013). A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells. Journal of Biomolecular Screening 18, 797–806.; 3) Ding et al. (2015) Ratiometric biosensors based on dimerization dependent fluorescent protein exchange. Nature Methods, doi:10.1038/nmeth.3261
Additional Information:
VECTOR: BacMam; PROMOTER: CMV