Red PIP2 Sensor BacMam Kit (CMV promoter)

Live cell assay. PIP2 is a red, dimerization-dependent fluorescent protein sensor under the control of a CMV promoter. When PIP2 is hydrolyzed, red fluorescence decreases. Packaged in BacMam, a BSL-1 insect virus engineered with a mammalian promoter, for safe handling and expression in a wide range of cell types. This sensor can be multiplexed with our green sensors for cAMP or DAG. When co-expressed with a green dimerization dependent monomer, GA, this sensor becomes a fluorescent protein exchange (FPX) sensor that increases in green fluorescence as the red fluorescence decreases.