OVA (257-264)-KLH

OVA 257 264 peptide (SIINFEKL) conjugated to KLH: Ovalbumin (Uniprot P01012) is the major component of egg white. It has been demonstrated that Ovalbumin contains B-cell epitopes which are recognized by specific IgE antibodies and CD4 T cell epitopes restricted by the MHC I-Ad molecule in mice and by HLA-D molecule in human such as OVA(257-264). OVA 257-264 is used to stimulate T cells in PBMCs and to quantify peptide epitope specificity and IFN-γ releasing effector cells by ELISPOT assay. OVA 257-264 is also used to test new adjuvant in immunotherapeutic vaccine development. OVA 257-264 can form a stable hydrogel and stimulate a immune response. This reaction seems to be linked with OVA 257-264 property to self-assemble into a hydrogel. Keyhole limpet hemocyanin (KLH) is a carrier protein which enhances immune response against haptens conjugated to it. OVA257-264 conjugated to KLH elicits specific antibodies anti-SIINFEKL.