pGlu-Glu-Pro Amide

[Glu2]-TRH tripeptide applications: In the last few years, [Glu2]-TRH, or PGPA for pyroglutamyl-glutamyl-proline amide, has been widely studied due to its potential applications in science and medicine. Indeed, this synthetic peptide has been associated with a wide range of biological effects, including anti-inflammatory, antioxidant, and neuroprotective activities. Moreover, low levels of TSH being naturally impacted by deficient production of TRH, analogs with impaired recognition by thyroliberinase, such as [Glu2]-TRH, depicted interesting features to counteract its degradation. As a matter of fact, histidine substitution by glutamic acid in TRH original sequence prevents enzymatic cleavage between the N-terminal pyroglutamyl group and the usual central histidine residue. This metabolically stable synthetic peptide significantly attenuates TRH-induced hippocampal extracellular acetylcholine release, thereby acting as a negative modulator of TRH cholinergic effect in mice brain.