Spin Trapping

Spin traps react with free radicals in solution to yield stable adducts which can be observed by EPR spectroscopy. The nitrone spin trap DMPO is widely used to detect free radicals, and is particularly useful for identifying oxygen-centered radicals like superoxide and hydroxyl radicals as well as C, N and S-centered radicals due to the characteristic EPR spectra of the adducts. BMPO is another useful spin trap to detect and identify the structure of reactive oxygen-centered radicals with a significantly longer half-life of its superoxide radical adduct. Both spin traps (DMPO and BMPO) are cell permeable making them useful for detecting extracellular and intracellular reactive oxygen species in vivo.

DMPO

Application: EPR (ESR) detection, immuno-spin trapping method

Ultra high purity
No pre-purification required

DMPO is provided at high purity and does not require any pre-purification as compared to most commercially available DMPO which require further purification to remove impurities that cause high backgrounds.

Figure 1: ESR spectra were taken in the presence of hydroxy radicals generated by Fenton reaction (black) and blank (blue). Cat# D048-10 gives very clear signal and higher S/N ratio compared to DMPO from two common suppliers.

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BMPO

Application: EPR (ESR) detection, superoxide anion radical

Longer half-life versus other reagents
High solubility in water
Greater S/N ratio

BMPO detects both superoxide and hydroxyl radicals and shows a much longer half-life (t_1/2=24 min) than other spin trap reagents.

Figure 2: Half-life of BMPO. BMPO is applicable for experiments which require longer handling times (ex. enzyme reactions)

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