Mayflower Bioscience offers access to a karyotype analysis service for both mouse and human cells. Karyotype analysis is a cytogenetic test that enables the identification of numerical and structural chromosomal abnormalities. Using conventional Giemsa staining techniques, condensed chromosomes are G-banded for observation. Chromosomes are subsequently grouped according to their size, centromere position and banding pattern, and any potential aberrations are identified. Karyotype G-banding is the gold standard to identify a range of gross chromosomal abnormalities with Mb resolution. Unlike array-based analysis, only karyotype G-banding provides an assessment of all these important characteristics of cells.
The service results consist of a descriptive report and at least one high-quality image of a representative karyotype.
For all services, we aim to provide karyotype analysis of 20 G-banded metaphase spreads from a single cell culture sample. However, if fewer than 20 cells are found in the sample provided, as many cells as possible will be analyzed.
For complex mosaic karyotypes, fewer than 20 cells may be analyzed and additional karyogram images will be provided in the report. Please note that for cancer cells, and in any cases where very complex, unstable karyotypes are observed, the analysis consists only of a full karyotype for a single cell. If further analysis is required, additional charges will apply.
The results are returned by email within 15–17 business days for human samples and within 20–22 business days for mouse samples. Please note that lead times can be longer during busier periods. If this applies to your order, our team will keep you updated.
Types of service
We provide analysis of both mouse and human cells. Other species are available on request, e.g. rat or pig.
For human and mouse samples, there are two services available:
- Fixed cells –cells already processed and fixed in the researchers’ laboratories. A complete and detailed protocol is provided.