Exosome Detection
TRIFic™ Exosome Assays
TRIFic™ exosome assays quickly provide quantitative data from purified or unpurified samples, including direct measurement of exosomes in plasma. TRIFic exosome assays are being developed for widely used markers of exosomes; the tetraspanin proteins CD9, CD63 and CD81. Kits come complete including control exosomes for standard curves. TRIFic exosome assays deliver clear, consistent data for the following reasons:
- Simplicity of the assay results in a high degree of reproducibility.
- Europium is used for detection, providing a high degrees of sensitivity.
- Only antigens held in association in multiple copies (such as tetraspanins in exosomes) are detectable with the assay, resulting in specificity.
TRIFic Exosome Assay Schematic

Step 1: Biotinylated antibody is bound to streptavidin coated assay plates.
Step 2: Biological samples are added. Exosomes and any free antigen are captured by the antibody.
Step 3: Europium labeled antibody is added and binds specically to exosome antigen. Samples are read in a time resolved fluorescence plate reader.
Readings obtained using the TRIFic exosome assay with the provided standard (exosomes purified from LNCaP cells). Note the linearity of response over a wide range of concentrations.
Products
Note: product availability depends on country. Product detail pages show availability of each product.
TRIFic™ Exosome Assay, human CD9
TRIFic™ Exosome Assay, human CD63
TRIFic™ Exosome Assay, human CD81

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