Calcium Transient Assay

The Calcium Transient Assay facilitates the rapid screening of drug compounds for cardiac arrhythmogenic and contraction liabilities by measuring calcium release and re-uptake from the sarcoplasmic reticulum within adult hiPSC cardiomyocytes in 96 well and 384 well plates.

Figure 1: Internal calcium traces from hIPSC cells exposed to isoproterenol. A. Baseline record shows the cells beating at 16 beats per minute. B. The cells are beating at 28 beats per minute after 10 nM isoproterenol. The rate of beating, the amplitude of the calcium released and the rate of internal calcium reuptake has increased significantly with isoproterenol treatment.

Figure 2: Internal calcium traces from hIPSC cells exposed to carbachol. A. Baseline record shows the cells beating at 16 beats per minute. B. The cells are beating at 10 beats per minute after exposure to 10 nM carbachol. The rate of beating decreased significantly with carbachol treatment.