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iclines

Action Potential Dose Response

Electrophysiology assay to evaluate compound effects on adult human cardiac action potentials. The assay is performed on ventricular-like iCell® Cardiomyocytes or Cor.4U® Cardiomyocytes at physiological temperature. A minimum of four cells are tested per compound. Further evaluation of active compounds on individual ion channels is available. 
 
Standard Acute Protocol
  • Control recording at baseline
  • Record during separate test doses
Measurement Parameters
  • Resting Membrane Potential (mV)
  • Action Potential Amplitude (mV)
  • Action Potential Upstroke Velocity (V/s)
  • Action Potential Duration (ms)
  • Rate (BPM - beats per minute)

acute

A. Action potential recording from a single adult human iPSC cardiomyocyte exposed to increasing concentrations of the hERG blocker E-4031. E-4031 significantly prolonged the action potential in a dose-dependent manner.

B. Action potential recording of a single adult human iPSC cardiomyocyte exposed to increasing concentrations of verapamil. The lowest dose, 0.1 μM, caused a significant shortening of the action potential due to blockade of the L-type calcium channel; but at a higher dose, 3.0 μM verapamil, the shortening of the action potential duration was reversed due to blockade of the hERG channel.

 
 
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