Ligand-gated Ion Channel Cell Lines

Acetylcholine

Target	Gene	Species	Cell Type	Expression
nAChR α4β2	CHRNA4 / CHRNB2	human	CHO	constitutive

GABA_A

Target	Gene	Species	Cell Type	Expression
GABAA α1β2γ2	GABRA1 / GABRB2 / GABRG2	human	CHO	constitutive
GABAA α2β2γ2	GABRA2 / GABRB2 / GABRG2	human	HEK 293	inducible

Glutamate

Target	Gene	Species	Cell Type	Expression
Kainate K2	GRIK2	human	CHO	constitutive
Kainate K2	GRIK2	human	HEK 293	inducible
NMDAR1	GRIN1	human	HEK 293	inducible
NMDA (NR1/2A)	GRIN1 / GRIN2A	human	HEK 293	inducible
NMDA (NR1/2B)	GRIN1 / GRIN2B	human	HEK 293	inducible
NMDA (NR1/2D)	GRIN1 / GRIN2D	human	HEK 293	inducible

Glycine

Target	Gene	Species	Cell Type	Expression
GlyRα1	GLRA1	human	CHO	constitutive
GlyRα2	GLRA2	human	CHO	constitutive
GlyRα3	GLRA3	human	HEK 293	inducible
GlyRα3	GLRA3	rat	CHO	constitutive
GlyRα3	GLRA3	human	CHO	constitutive

Serotonin

Target	Gene	Species	Cell Type	Expression
5HT3A	5HT3A	human	CHO	constitutive

Acid Sensing

Target	Gene	Species	Cell Type	Expression
ASIC1a long	ACCN2	human	CHO	constitutive
ASIC1a short	ACCN2	human	CHO	constitutive
ASIC1b	ACCN2	human	CHO	constitutive
ASIC2	ACCN1	human	CHO	constitutive

P2X

Target	Gene	Species	Cell Type	Expression
P2X1	P2rx1	human	CHO	constitutive
P2X2	P2rx2	human	CHO	constitutive
P2X2/3	P2rx2/P2rx3	human	CHO	constitutive
P2X3	P2rx3	human	CHO	constitutive
P2X4	P2rx4	human	HEK 293	constitutive
P2X7	P2rx7	human	HEK 293	constitutive