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Exo-spin™ midi Columns (EX04 for larger concentrated samples)

For the direct purification of sample sizes up to 1 ml. Larger sample volumes can be used with prior precipitation.
 
exomidi
 
Exo-spin™ midi columns are a versatile, quick and effective way to isolate and purify exosomes directly without precipitation from a range of samples like blood sera and cell culture medium. No precipitation is required for volumes up to 1ml making these columns an ideal choice for applications like mass spec which is not compatible with the use of precipitants. Cell debris, proteins and other impurities are effectively removed yielding a clean exosome sample in less than 30 minutes.
 

Publication

Brook AC, Jenkins RH, Clayton A, Kift-Morgan A, Baby AC, Shephard AP, Mariotti B, Cuff SM, Bazzoni F, Bowen T, Fraser DJ, and Eberl M. (2019). Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis. Scientific Reports 9(1):10136. 

Salimu J, Webber J, Gurney M, Al-Taei S, Clayton A and Tabi Z. (2017). Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes. Journal of Extracellular Vesicles 6(1):1368823.

Santangelo L, Giurato G, Cicchini C, Montaldo C, Mancone C, Tarallo R, Battistelli C, Alonzi T, Weisz A and Tripodi M. (2016). The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. Cell Reports 17(3):799-808.

Welton JL, Webber JP, Botos LA, Jones M and Clayton A. (2015). Ready-made chromatography columns for extracellular vesicle isolation from plasma. Journal of Extracellular Vesicles 4:27269

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Exo-Spin Midi Columns EX04-5 · Cell Guidance Systems
EX04-5
Cell Guidance Systems
5 columns $210.00
5 columns
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Exo-Spin Midi Columns EX04-20 · Cell Guidance Systems
EX04-20
Cell Guidance Systems
20 columns $615.00
20 columns
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Exo-spin Buffer EX06-250 · Cell Guidance Systems
EX06-250
Cell Guidance Systems
250 ml $245.00
250 ml
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