Exo-spin™ 96 (EX07 for blood sera and plasma)

Exo-spin™ 96 provides proven size exclusion chromatography (SEC) mini-column (EX01) technology in a 96-well format, allowing up to 96 individual samples to be processed in less than 30 minutes. Sample volumes up to 0.5 ml can be purified. 

The kit provides flexibility with 8-column strips detachable from the plate, so you can process 8 samples in 8-column strips if needed 

The Exo-spin™ 96 well product offers:

• High-throughput exosome isolation at the benchtop in the standard 96 well format in under 30 minutes
• Purify up to 0.5 ml of each sample
•  >97% of free proteins removed
• Outstanding reproducibility +/- 5% Standard deviation, for consistent exosome purification, time after time
• Flexibility to just work with single strips of 8 columns at a time
• The convenience of purified exosomes eluted directly into a 96 well plate ready for high throughput downstream applications

The Exo-spin™ 96 can be used to isolate exosomes directly from protein-rich samples such as sera and plasma. If required, you can concentrate the sample with your preferred concentration method (e.g. concentrator, precipitation buffer)  to isolate from large volumes of low-protein biofluids, such as cell culture medium, cerebral spinal fluid (CSF), and urine.

The EX07 Exo-spin™ kit has been developed to process up to 500 µl blood samples (plasma or sera) using iterative loading.

Downstream applications: advice and content

The EX07 Exo-spin™ 96 kit is compatible with all downstream application and has been published in a large range of different applications.

RNA analysis:

• Wang L., Wang C., Jia X. and Yu J. (2018). Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis. Cellular Physiology and Biochemistry; 50:1754–1763.

• Emanueli C., Shearn AI., Laftah A., Fiorentino F., Reeves BC., Beltrami C., Mumford A., Clayton A., Gurney M., Shantikumar S. and Angelini G. (2016). Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery. PLoS One 29;11(4):e0154274. 

Mass Spectrometry:

• Menezes-Neto A., Fidalgo Sáez MJ., Lozano-Ramos I., Segui-Barber J., Martin-Jaular L., Estanyol Ullate JM., Fernandez-Becerra C., Borrás FE., and del Portillo HA. (2015). Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. Journal of Extracellular Vesicles; 4: 10.3402/jev.v4.27378. 

Functional study:

• Sheller-Miller S, Trivedi J, Yellon SM, and Menon R. (2019). Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Scientific Reports 24;9(1):608.

Citations

1) Sheller-Miller S, Trivedi J, Yellon SM, and Menon R. (2019). Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Scientific Reports 24;9(1):608.

2) Soares Martins, T., Catita, J., Martins Rosa, I., Cruz da Silva, O., and Henriques, A.G. (2018) Exosome isolation from distinct biofluids using precipitation and column-based approaches. PLoS One. 13(6):e0198820.

3) Wang L., Wang C., Jia X. and Yu J. (2018). Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis. Cellular Physiology and Biochemistry; 50:1754–1763.

4) Emanueli C., Shearn AI., Laftah A., Fiorentino F., Reeves BC., Beltrami C., Mumford A., Clayton A., Gurney M., Shantikumar S. and Angelini G. (2016). Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery. PLoS One 29;11(4):e0154274

5) Menezes-Neto A., Fidalgo Sáez MJ., Lozano-Ramos I., Segui-Barber J., Martin-Jaular L., Estanyol Ullate JM., Fernandez-Becerra C., Borrás FE., and del Portillo HA. (2015). Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. Journal of Extracellular Vesicles; 4: 10.3402/jev.v4.27378.