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Exosome Tracking

Exosome Tracking

ExoFLARE™ Exosome Tracking Reagents

The ExoFLARE system are a set of transfection-ready vectors to transiently or stably express human tetraspanin proteins tagged with a unique protein tag (FLARE - FLuorescence Activating Response Element) in your cells of interest. FLARE-tagged proteins incorporated into exosomes are detected with a tag-specific fluorophore to allow tracking of exosome movement for extensive periods.

Neither the FLARE tag or dye fluoresce alone. When the FLARE protein and dye combine, a conformational change in the dye structure occurs resulting in fluorescence. There is continuous turnover of the dye for sustained fluorescence but without the levels of photo-bleaching associated with fluorescent proteins making the system ideal for long-term tracking. ExoFLARE dyes are offered in both cell permeable and cell impermeable formats offering flexibility in experimental design.

For long-term tracking of exosomes, use ExoFLARE™ to:

  • Image individual exosomes
  • Track long term movement of exosomes
  • Detect uptake of exosomes in non-transfected recipient cells

Key Attributes

  • Significantly brighter than fluorescent proteins
  • Differentiate between donor and recipient cell exosomes
  • Low background levels
  • Non-toxic
exosometracking

Figure 1: ExoFLARE constructs were transiently transfected into DU154N cells. ExoFLARE cell-permeable dye was added to the media and cells were imaged using a confocal fluorescence microscope. Red = ExoFLARE; Green = Hoechst.

ExoFLARE Kits

Construct

CD9

CD63

CD81

With Cell Permeable Dye

EX304

EX305

EX306

With Cell Impermeable Dye

EX301

EX302

EX303

Fluorophores

  • Cell permeable
  • Cell impermeable

Products

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