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cADDis cAMP Assay

cADDis cAMP Assay

the brightest live cell assay in the world for measuring cAMP

cADDis cAMP Assay is a simple and robust assay for Gs and Gi signaling without the need for co-factors, cell lysis, or complex liquid handling. cADDis is genetically-encoded and packaged in BacMam for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers. These fluorescent sensors can be combined with different colored sensors to measure multiple signals simultaneously.

cADDis cAMP Assays are offered as either:

  • Upward Assays (cAMP increase, fluorescence signal increase)
  • Downward Assays (cAMP increase, fluorescence signal decrease)

Downward assays are recommended for conditions of high background fluorescence to more easily separate signal from background.

Gs detection: Kinetics. Dose responses. Bright and reliable signals.

Measure kinetics and dose responses of Gs-mediated cAMP production with large and consistent fluorescence changes. cADDis is extremely bright with remarkable signal to noise (Z’ > 0.95). 

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Isoproterenol induced cAMP production: Kinetics, Dose Response, and Assay Reliability cADDis in live HEK293 cells stimulated with isoproterenol, a selective agonist of ß-adrenergic receptors. Bottom: fluorescence images before and after Iso addition.

Gi detection: Forskolin-free

cADDis detects Gi-mediated decreases in cAMP production, in real time, without the need for forskolin or IBMX. The cADDis Gi assay yields Z’ values > 0.85. Measure biologically relevant Gi signaling, without the need for forskolin, using cADDis.

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Gi signaling: Kinetics, Dose Response, and Assay Reliability  cADDis in live HEK293 cells stimulated with quinpirole, a selective agonist of dopaminergic D2 receptors.

Discover cADDis today

Inexpensive trial kit are available. Custom packaging in lentivirus and AAV available upon request.

Products

Note: product availability depends on country. Product detail pages show availability of each product.

Applications: live cell assay, cAMP levels
Applications: live cell assay, cAMP levels
Applications: live cell assay, cAMP levels
Applications: live cell assay, cAMP levels
Applications: live cell assay, cAMP levels
Applications: live cell assay, cAMP levels
Applications: live cell assay, cAMP levels