RNA Reprogramming Systems designed for reproducible and efficient generation of iPS cell lines from both normal and patient-specific cells. Two RNA Reprogramming options are available for IPSC generation: 1) mRNA only reprogramming and 2) microRNA-enhanced mRNA reprogramming.

mRNA Only Reprogramming

• mRNA Reprogramming Kit, Cat No.: 00-0071
• RNA Transfection Kit, Cat No.: 00-0069

The mRNA Reprogramming Kit includes synthetically modified mRNAs and soluble B18R to attenuate TLR- and Type I IFN signaling associated with innate, antiviral immunity. Pluriton™ Reprogramming Medium is included and is specifically designed to support mRNA based delivery and reprogramming. The kit is formatted to allow tunable cellular expression of individual reprogramming factors transfected into target cells, providing researchers the flexibility to choose the optimal conditions for their experiment. Standalone mRNA sets and individual mRNAs are also available.

microRNA-enhanced mRNA Reprogramming

• mRNA Reprogramming Kit, Cat No.: 00-0071
• microRNA Booster Kit, Cat No.: 00-0073
• RNA Transfection Kit, Cat No.: 00-0069

The microRNA-enhanced mRNA Reprogramming method delivers faster reprogramming kinetics, a streamlined protocol and improved efficacy on hard-to-reprogram or refractory cell types. The mRNA Reprogramming Kit in combination with the microRNA Booster Kit and the RNA Transfection Kit generates expandable primary iPS cell colonies in as little as 8 days. This new method improves kinetics and reduces mRNA dose per transfection resulting in a per well cost reduction of approximately 35% to 45% versus feeder-based mRNA Reprogramming.

Products

Note: product availability depends on country. Product detail pages show availability of each product.