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RNAiONE - shRNA design and validation

RNAiONE - shRNA design and validation
Fields of Application 
Identification of optimal shRNA sequences 
for knockdown cell systems 
for target validation 
screening applications 
 
Project Statistics & Customer Satisfaction Guarantee
Over the years SIRION BIOTECH has consisitently proven its technology: in 80% of recent projects they have managed to identify shRNAs with knockdown results of above 80%. This experience makes it possible for them to issue warranties with their quotes. 
 
Principle
Cloning of the cDNA of Your Gene of Interest (GOI) into the pVal vector (=pVal-GOI)
Cloning of 5, 10, 15 or more different shRNAs into pVal-GOI (=pVal-GOI-shRNA 1-x)
Transfection of 293 or NIH-3T3 cells with the resulting validation vectors 
Quantification of target gene knockdown by qRT-PCR  relative to control cells
 
To receive a price quotation, email a description of your project specifications to sales@mayflowerbio.com.
 
rnaione
 
Figure: EGFP-GPCRx expression in NIH-3T3 cells transfected with plasmids shRNA sequences (1-15) or not-target (NT) shRNA.

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