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qPCR Detection Kits, meat speciation

qPCR Detection Kits, meat speciation
genesig Meat Speciation Kits provide a method for detecting species-specific mitochondrial DNA that may be present in a food sample by PCR amplification. PCR amplification is detected by means of a hydrolysis probe (“Taqman-style”) which is degraded during PCR, releasing fluorescence. The fluorescence trace can be used to both detect and quantify the number of copies of mitochondrial DNA present in the sample. 
 
Sensitivity 
Under optimal PCR conditions the kit provides exceptional sensitivity. Priming efficiency is guaranteed >95% and the kit can detect less than 100 copies of the target mtDNA, or an estimated 1-2 muscle cells within a sample. 
 
Specificity 
The kit provides species specific primers and probe that detect the species of interest. The primers and probe are read through the FAM channel and detect the presence of the mitochondrial DNA belonging to that particular species.

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