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PCR Product Clean-Up

PCR Product Clean-Up
For years, Shrimp Alkaline Phosphatase (SAP) has been utilized together with Exonuclease I to offer a simple and effective method for removing nucleotides and primers from PCR products prior to sequencing or genotyping. SAP dephosphorylates nucleotides and ExoI degrades primers which interfere with the primer extension reaction. SAP can be fully inactivated by a short heat treatment.
 
Advantages
  • 100% heat-inactivated at 65°C
  • Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
  • Works in restriction enzyme buffers
  • Used in PCR clean-up
 
Simple Protocol
Add the following to your PCR reactions after PCR is completed in preparation for sequencing or genotyping:
  • 2 Units SAP
  • 10 Units Exonuclease I
  • Incubate at 37°C for 15 min
  • Inactivate at 80°C for 15 min
 
Disclaimer
Certain applications of ArcticZymes AS products may require licenses from others. It is the expressed duty of any receiver of these products to acquire such licenses, if necessary. In no event shall Mayflower Bioscience or its supplier, ArcticZymes AS, be liable for claims for any damages, whether direct, incidental, foreseeable, consequential, or special (including but not limited to loss of use, revenue or profit), arising due to the violation of third parties Intellectual Property Rights by any receiver of ArcticZymes AS products. ArcticZymes AS products may be covered by pending or issued patents, designs or design applications and/or trademarks or trademark applications or any other registered or unregistered Intellectual Property Right.

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