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PCR Product Clean-Up

PCR Product Clean-Up
For years, Shrimp Alkaline Phosphatase (SAP) has been utilized together with Exonuclease I to offer a simple and effective method for removing nucleotides and primers from PCR products prior to sequencing or genotyping. SAP dephosphorylates nucleotides and ExoI degrades primers which interfere with the primer extension reaction. SAP can be fully inactivated by a short heat treatment.
  • 100% heat-inactivated at 65°C
  • Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
  • Works in restriction enzyme buffers
  • Used in PCR clean-up
Simple Protocol
Add the following to your PCR reactions after PCR is completed in preparation for sequencing or genotyping:
  • 2 Units SAP
  • 10 Units Exonuclease I
  • Incubate at 37°C for 15 min
  • Inactivate at 80°C for 15 min
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