Assay Specifications
Sample types: cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): majority of the assays < 10 pg/ml
Quantitation range (majority of assays): LLOQ: < 20 pg/mL; ULOQ: > 5,000 pg/ml
Standard dose recovery: 70-130%
Precision: Intra-assay CV: < 10%; Inter-assay CV: < 20%
Cross-reactivity of analytes in a panel or group: Negligible
Sample volume: 15 µl
Correlation with BD™ CBA
Human normal and abnormal serum samples were measured with both AimPlex and CBA assays and analyzed on a BD FACSCanto II.
Correlation with xMAP®
Cell culture supernatant samples from human PBMCs treated with various reagents were assayed by using AimPlex human Th1/2/17 7-Plex assays and a commercially available Luminex multiplex assay kit. AimPlex Assays were analyzed on a BD FACSCalibur
Representative Data
Serum samples from mice, treated with 5mg/kg of lipopolysaccharides (LPS 1 and 2) or without (Control 1 and 2) for 6 hours, were tested using an AimPlex mouse 16-plex panel. Analyzed on a BD FACSCalibur. (data provided by Dr. ZX Liu, USC)
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